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Importing Data from GenePix Files

 

Overview

The data files must be in the Axon .gpr file format.

 

Sample Order

The sample order of imported datasets is determined by the order of the source sample data files listed in the Import Data dialog.

 

Template

Result of Import

GenePix

Multiple files are processed into a single dataset.

GenePix Merge Replicates

Multiple files are processed into a single dataset.

GenePix Green/Red

Multiple files are processed into a single ratio dataset (treatment/control).

GenePix Red/Green

Multiple files are processed into a single ratio dataset (treatment/control).

 

If you are importing using one of the two-color data templates (the dye colors are listed as treatment/control in the template name), all data values <0 are replaced with missing values (null values). Between-chip replicate measurements are imported as samples with the same names.

When the import process is complete, a dataset that is the ratio of treatment/control is added to the Experiments navigator. A selected sample ratio can be displayed in an intensity-bias plot to determine whether Lowess normalization is appropriate for the dataset.

 

Import Process for GenePix and GenePix Merge Replicates

Import Process for GenePix Green/Red

Import Process for GenePix Red/Green

 

Related Topics:

Selecting a Template for Data Import

Importing Multiple Files With One Sample Each

Two-Color Data

Merging Within-Chip Replicate Measurements