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Creating an Intensity-Bias Plot of a Sample Ratio



An intensity-bias plot can be used to view dye biases to determine whether normalization is required. An intensity-bias plot is a scatter plot of the log-ratio versus the log-intensity.



1. Click a two-color dataset in the Experiments navigator. The item is highlighted.

2. Click the Table View toolbar icon , or select Table View from the Data menu, or right-click the item and select Table View from the shortcut menu. A table view of the dataset is displayed.

3. Click on the name of a sample. The sample is highlighted.

4. Select Intensity-Bias Plot from the Explore menu. An intensity-bias plot of the highlighted sample is displayed.


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Subtraction of Central Tendency