Gene expression values are normalized by subtracting the mean, followed by dividing the standard deviation for each gene.
This procedure standardizes each gene. The mean and standard deviation for each gene is calculated, and each value for the gene (x) is standardized using (x -mean)/standard deviation.
1. Click a complete dataset in the Experiments navigator. The item is highlighted.
2. Click the Normalize toolbar icon , or select Normalize from the Data menu, or right-click the item and select Normalize from the shortcut menu. The first Normalization dialog is displayed.
3. Double-click the Other Transformations radio button, or click it and click Next. The second Normalization dialog is displayed.
4. Double-click the Standardize radio button, or click it and click Finish. The Experiment Progress dialog is displayed. It is dynamically updated as the Standardize Normalization operation is performed. To cancel the Standardize Normalization operation, click the Cancel button.
If the operation cannot complete an error message is displayed. The operation will fail, for example, if the standard deviation of a gene is zero.
Upon successful completion, a new normalization dataset is added under the original dataset in the Experiments navigator.