Gene expression values are normalized by dividing each value for a gene by the maximum value observed in any sample for that gene.
1. Click a complete dataset in the Experiments navigator. The item is highlighted.
2. Click the Normalize toolbar icon , or select Normalize from the Data menu, or right-click the item and select Normalize from the shortcut menu. The first Normalization dialog is displayed.
3. Double-click the Other Transformations radio button, or click it and click Next. The second Normalization dialog is displayed.
4. Double-click the Divide by Maximum radio button, or click it and click Finish. The Experiment Progress dialog is displayed. It is dynamically updated as the Divide by Maximum Normalization operation is performed. To cancel the Divide by Maximum Normalization operation, click the Cancel button.
If the operation cannot complete an error message is displayed. The operation will fail, for example, if the maximum of a gene is zero.
Upon successful completion, a new normalization dataset is added under the original dataset in the Experiments navigator.